Human preadipocytes cultured with AcMC — induced ECM and inflammation related genes

ECM component

Gene ID

Symbol

Name

Fold variation

Tissular fraction

ECM role

Structural proteins

 

1292

COL6A2

collagen, type VI, alpha 2

3.68

SVF

cell matrix adhesion

1278

COL1A2

collagen, type I, alpha 2

3.47 SVF proper matrix

1291

COL6A1

collagen, type VI, alpha 1

1.86 SVF cell matrix adhesion

1290

COL5A2

collagen, type V, alpha 2

1.64 SVF proper matrix

84034

EMILIN2

elastin microfibril interfacer 2

1.45 SVF proper matrix

1293

COL6A3

collagen, type VI, alpha 3

1.23 proper matrix

1277

COL1A1

collagen, type I, alpha 1

0.89 SVF proper matrix

81578

COL21A1

collagen, type XXI, alpha 1

0.84 SVF proper matrix

1303

COL12A1

collagen, type XII, alpha 1

0.67 proper matrix

1282

COL4A1

collagen, type IV, alpha 1

0.61 SVF proper matrix

1306

COL15A1

collagen, type XV, alpha 1

0.43 SVF proper matrix

2006

ELN

elastin (supravalvular aortic stenosis, Williams-Beuren syndrome)

0.26 SVF proper matrix

Glycoproteins

3959

LGALS3BP

lectin, galactoside-binding, soluble, 3 binding protein

8.00 Adipocyte cell cell adhesion

3678

ITGA5

integrin, alpha 5 (fibronectin receptor, alpha polypeptide)

7.18 cell matrix adhesion

3956

LGALS1

lectin, galactoside-binding, soluble, 1 (galectin 1)

3.95 cell matrix adhesion

8434

RECK

reversion-inducing-cysteine-rich protein with kazal motifs

3.42 remodeling

3913

LAMB2

laminin, beta 2 (laminin S)

3.31 cell matrix adhesion

3998

LMAN1

lectin, mannose-binding, 1

3.10 cell cell adhesion

3684

ITGAM

integrin, alpha M (complement component 3 receptor 3 subunit)

2.85 SVF cell matrix adhesion

7058

THBS2

thrombospondin 2

2.54 SVF cell matrix adhesion

946

SIGLEC6

sialic acid binding Ig-like lectin 6

2.49 cell cell adhesion

3964

LGALS8

lectin, galactoside-binding, soluble, 8 (galectin 8)

2.42 SVF cell cell adhesion

2335

FN1

fibronectin 1

2.28 SVF cell matrix adhesion

1012

CDH13

cadherin 13, H-cadherin (heart)

1.95 SVF cell cell adhesion

81562

LMAN2L

lectin, mannose-binding 2-like

1.73 SVF cell cell adhesion

3912

LAMB1

laminin, beta 1

1.59 cell matrix adhesion

9358

ITGBL1

integrin, beta-like 1 (with EGF-like repeat domains)

1.53 cell matrix adhesion

22795

NID2

nidogen 2 (osteonidogen)

1.51 SVF cell matrix adhesion

960

CD44

CD44 molecule (Indian blood group)

1.44 SVF cell matrix adhesion

3685

ITGAV

integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

1.25 Adipocyte cell matrix adhesion

3909

LAMA3

laminin, alpha 3

1.21 Adipocyte cell matrix adhesion

3689

ITGB2

integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)

1.19 SVF cell matrix adhesion

3908

LAMA2

laminin, alpha 2 (merosin, congenital muscular dystrophy)

1.06 SVF cell matrix adhesion

3910

LAMA4

laminin, alpha 4

1.05 SVF cell matrix adhesion

7059

THBS3

thrombospondin 3

0.40 SVF cell matrix adhesion

7123

CLEC3B

C-type lectin domain family 3, member B

0.38 SVF cell cell adhesion

10631

POSTN

periostin, osteoblast specific factor

0.34 SVF cell matrix adhesion

8506

CNTNAP1

contactin associated protein 1

0.32 SVF cell matrix adhesion

Glycosaminoglycans and proteoglycans

4060

LUM

lumican

2.86 SVF proper matrix

145864

HAPLN3

hyaluronan and proteoglycan link protein 3

2.81 cell matrix adhesion

51604

PIGT

phosphatidylinositol glycan anchor biosynthesis, class T

2.41 remodeling

1758

DMP1

dentin matrix acidic phosphoprotein

1.97 cell matrix adhesion

6443

SGCB

sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)

1.90 Adipocyte cell matrix adhesion

6383

SDC2

syndecan 2 (heparan sulfate proteoglycan 1, cell surface-associated, fibroglycan)

1.41 cell matrix adhesion

10026

PIGK

phosphatidylinositol glycan anchor biosynthesis, class K

1.33 remodeling

9672

SDC3

syndecan 3 (N-syndecan)

0.76 SVF cell matrix adhesion

1605

DAG1

dystroglycan 1 (dystrophin-associated glycoprotein 1)

0.66 SVF cell matrix adhesion

2719

GPC3

glypican 3

0.64 SVF cell matrix adhesion

5281

PIGF

phosphatidylinositol glycan anchor biosynthesis, class F

0.63 remodeling

6695

SPOCK1

sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1

0.60 SVF proper matrix

6386

SDCBP

syndecan binding protein (syntenin)

0.55 cell cell adhesion

Enzymes

4314

MMP3

matrix metallopeptidase 3 (stromelysin 1, progelatinase)

37.99 remodeling

4312

MMP1

matrix metallopeptidase 1 (interstitial collagenase)

22.76 Adipocyte remodeling

7076

TIMP1

TIMP metallopeptidase inhibitor 1

8.10 SVF remodeling

7980

TFPI2

tissue factor pathway inhibitor 2

7.06 SVF remodeling

4318

MMP9

matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

7.06 SVF remodeling

8974

P4HA2

procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II

5.42 remodeling

5118

PCOLCE

procollagen C-endopeptidase enhancer

4.89 SVF remodeling

5351

PLOD1

procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1

3.49 Adipocyte remodeling

4313

MMP2

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

3.49 SVF remodeling

91039

DPP9

dipeptidyl-peptidase 9

3.27 remodeling

649

BMP1

bone morphogenetic protein 1

3.21 remodeling

3073

HEXA

hexosaminidase A (alpha polypeptide)

3.14 SVF remodeling

23169

SLC35D1

solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1

2.65 remodeling

1368

CPM

carboxypeptidase M

2.63 Adipocyte remodeling

6484

ST3GAL4

ST3 beta-galactoside alpha-2,3-sialyltransferase 4

2.48 remodeling

8751

ADAM15

ADAM metallopeptidase domain 15 (metargidin)

2.34 SVF remodeling

4016

LOXL1

lysyl oxidase-like 1

2.34 SVF remodeling

29906

ST8SIA5

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5

2.29 remodeling

55790

ChGn

chondroitin beta1,4 N-acetylgalactosaminyltransferase

2.24 remodeling

54187

NANS

N-acetylneuraminic acid synthase (sialic acid synthase)

2.01 SVF remodeling

10585

POMT1

protein-O-mannosyltransferase 1

2.00 remodeling

4247

MGAT2

mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase

1.95 SVF remodeling

5328

PLAU

plasminogen activator, urokinase

1.90 SVF remodeling

6184

RPN1

ribophorin I

1.88 remodeling

23324

MAN2B2

mannosidase, alpha, class 2B, member 2

1.85 remodeling

23213

SULF1

sulfatase 1

1.85 SVF remodeling

1520

CTSS

cathepsin S

1.80 SVF remodeling

55501

CHST12

carbohydrate (chondroitin 4) sulfotransferase 12

1.73 SVF remodeling

4316

MMP7

matrix metallopeptidase 7 (matrilysin, uterine)

1.67 SVF remodeling

8705

B3GALT4

UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4

1.56 remodeling

6480

ST6GAL1

ST6 beta-galactosamide alpha-2,6-sialyltranferase 1

1.55 SVF remodeling

6868

ADAM17

ADAM metallopeptidase domain 17 (tumor necrosis factor, alpha, converting enzyme)

1.48 SVF remodeling

8722

CTSF

cathepsin F

1.45 Adipocyte remodeling

7077

TIMP2

TIMP metallopeptidase inhibitor 2

1.45 SVF remodeling

8754

ADAM9

ADAM metallopeptidase domain 9 (meltrin gamma)

1.42 SVF remodeling

7113

TMPRSS2

transmembrane protease, serine 2

1.40 remodeling

201595

STT3B

STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)

1.31 remodeling

53616

ADAM22

ADAM metallopeptidase domain 22

1.30 SVF cell matrix adhesion

2135

EXTL2

exostoses (multiple)-like 2

1.30 SVF remodeling

9215

LARGE

like-glycosyltransferase

1.29 SVF remodeling

5034

P4HB

procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide

1.28 remodeling

9514

GAL3ST1

galactose-3-O-sulfotransferase 1

1.26 SVF remodeling

54878

DPP8

dipeptidyl-peptidase 8

1.25 remodeling

8869

ST3GAL5

ST3 beta-galactoside alpha-2,3-sialyltransferase 5

1.24 SVF remodeling

951

CD37

CD37 molecule

1.23 SVF remodeling

1509

CTSD

cathepsin D (lysosomal aspartyl peptidase)

1.20 Adipocyte remodeling

126792

B3GALT6

UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6

1.20 SVF remodeling

8038

ADAM12

ADAM metallopeptidase domain 12 (meltrin alpha)

1.14 Adipocyte remodeling

8985

PLOD3

procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3

1.12 SVF remodeling

411

ARSB

arylsulfatase B

1.09 remodeling

4248

MGAT3

mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase

1.09 SVF remodeling

5329

PLAUR

plasminogen activator, urokinase receptor

1.07 SVF remodeling

5518

PPP2R1A

protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform

1.05 cell matrix adhesion

8728

ADAM19

ADAM metallopeptidase domain 19 (meltrin beta)

0.84 SVF remodeling

9488

PIGB

phosphatidylinositol glycan anchor biosynthesis, class B

0.74 Adipocyte remodeling

9510

ADAMTS1

ADAM metallopeptidase with thrombospondin type 1 motif, 1

0.74 SVF remodeling

2132

EXT2

exostoses (multiple) 2

0.72 remodeling

2990

GUSB

glucuronidase, beta

0.70 SVF remodeling

79716

NPEPL1

aminopeptidase-like 1

0.67 Adipocyte remodeling

8702

B4GALT4

UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4

0.66 remodeling

2517

FUCA1

fucosidase, alpha-L- 1, tissue

0.60 SVF remodeling

43

ACHE

acetylcholinesterase (Yt blood group)

0.52 cell cell adhesion

9951

HS3ST4

heparan sulfate (glucosamine) 3-O-sulfotransferase 4

0.48 Adipocyte remodeling

115209

OMA1

OMA1 homolog, zinc metallopeptidase (S. cerevisiae)

0.27 remodeling

4249

MGAT5

mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase

0.24 remodeling

81849

ST6GALNAC5

ST6 (alpha-N-acetyl-neuraminyl-2,3-beta- galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5

0.12 SVF remodeling

Other components

83716

CRISPLD2

cysteine-rich secretory protein LCCL domain containing 2

4.01 SVF cell cell adhesion

3371

TNC

tenascin C (hexabrachion)

3.57 SVF cell matrix adhesion

2152

F3

coagulation factor III (thromboplastin, tissue factor)

3.51 remodeling

3491

CYR61

cysteine-rich, angiogenic inducer, 61

3.42 Adipocyte cell cell adhesion

30008

EFEMP2

EGF-containing fibulin-like extracellular matrix protein 2

3.07 Adipocyte proper matrix

11082

ESM1

endothelial cell-specific molecule 1

2.50 proper matrix

6687

SPG7

spastic paraplegia 7, paraplegin (pure and complicated autosomal recessive)

2.45 cell matrix adhesion

11047

ADRM1

adhesion regulating molecule 1

2.38 cell matrix adhesion

4267

CD99

CD99 molecule

2.24 SVF cell matrix adhesion

6900

CNTN2

contactin 2 (axonal)

2.10 cell matrix adhesion

634

CEACAM1

carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)

2.10 cell matrix adhesion

1893

ECM1

extracellular matrix protein 1

2.06 SVF proper matrix

24145

PANX1

pannexin 1

2.06 SVF cell cell adhesion

682

BSG

basigin (Ok blood group)

1.95 Adipocyte cell matrix adhesion

3383

ICAM1

intercellular adhesion molecule 1 (CD54), human rhinovirus receptor

1.86 SVF cell cell adhesion

6091

ROBO1

roundabout, axon guidance receptor, homolog 1 (Drosophila)

1.83 cell matrix adhesion

5649

RELN

reelin

1.76 cell matrix adhesion

2162

F13A1

coagulation factor XIII, A1 polypeptide

1.55 SVF remodeling

131566

DCBLD2

discoidin, CUB and LCCL domain containing 2

1.50 cell matrix adhesion

9019

MPZL1

myelin protein zero-like 1

1.50 SVF cell matrix adhesion

8839

WISP2

WNT1 inducible signaling pathway protein 2

1.33 SVF cell matrix adhesion

2697

GJA1

gap junction protein, alpha 1, 43kDa (connexin 43)

1.32 SVF cell cell adhesion

11167

FSTL1

follistatin-like 1

1.21 SVF cell matrix adhesion

3385

ICAM3

intercellular adhesion molecule 3

0.86 cell cell adhesion

55591

VEZT

vezatin, adherens junctions transmembrane protein

0.72 cell cell adhesion

9223

MAGI1

membrane associated guanylate kinase, WW and PDZ domain containing 1

0.55 Adipocyte cell matrix adhesion

57165

GJA12

gap junction protein, alpha 12, 47kDa

0.44 SVF cell cell adhesion
 

8189

SYMPK

symplekin

0.40

Adipocyte

cell cell adhesion

Inflammatory molecules Gene ID Symbol Name Fold variation Tissular fraction  

Interferon family

8519 IFITM1 interferon induced transmembrane protein 1 (9-27) 5.02 SVF  
359948 IRF2BP2 interferon regulatory factor 2 binding protein 2 4.4 SVF  
3662 IRF4 interferon regulatory factor 4 2.41 Adipocyte  
3665 IRF7 interferon regulatory factor 7 3.67 SVF  
3429 IFI27 interferon, alpha-inducible protein 27 4.19 SVF  
2537 IFI6 interferon, alpha-inducible protein 6 3.11 SVF  

Interleukins

9173 IL1RL1 interleukin 1 receptor-like 1 1.73  
3598 IL13RA2 interleukin 13 receptor, alpha 2 4.51 Adipocyte  
11009 IL24 interleukin 24 6.4 SVF  
9235 IL32 interleukin 32 8.64 SVF  
259307 IL4I1 interleukin 4 induced 1 1.92  
3566 IL4R interleukin 4 receptor 3.01 SVF  
3569 IL6 interleukin 6 (interferon, beta 2) 6.46 SVF  
3575 IL7R interleukin 7 receptor 2.1 SVF  

Prostaglandins

9536 PTGES prostaglandin E synthase 5.23 SVF  
5743 PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) 14.1 SVF  

Acute phase proteins

6288 SAA1 serum amyloid A1 7.5  
6289 SAA2 serum amyloid A2 9.16 Adipocyte  
567 B2M beta-2-microglobulin 2.84 SVF  
6352 CCL5 chemokine (C-C motif) ligand 5 19.51 SVF  
9618 TRAF4 TNF receptor-associated factor 4 2.9 SVF  
7128 TNFAIP3 tumor necrosis factor, alpha-induced protein 3 2.19 SVF  
7130 TNFAIP6 tumor necrosis factor, alpha-induced protein 6 4.57 SVF  

Complement family

1380 CR2 complement component (3d/Epstein Barr virus) receptor 2 1.74  
715 C1R complement component 1, r subcomponent 2.51  
629 CFB complement factor B 4.1 SVF  

Others

151647 FAM19A4 family with sequence similarity 19 (chemokine (C-C motif)-like), member A4 3.33  
2191 FAP fibroblast activation protein, alpha 2.26 Adipocyte  
2247 FGF2 fibroblast growth factor 2 (basic) 2.84  
9446 GSTO1 glutathione S-transferase omega 1 3.17  
SVF = Stroma Vascular Fraction
The indicated fold variations were measured in the cDNA microarray experiments.