Obese WAT after gastric bypass — ECM related genes

ECM component

GeneID

Symbol

Name

Fold

variation

Tissular

fraction*

ECM role

Structural proteins

1277

COL1A1

collagen, type I, alpha 1

7.18

SVF

proper matrix

1278

COL1A2

collagen, type I, alpha 2

6.64

SVF

proper matrix

2006

ELN

elastin (supravalvular aortic stenosis, Williams-Beuren syndrome)

3.64

SVF

proper matrix

1293

COL6A3

collagen, type VI, alpha 3

3.28

—

proper matrix

84034

EMILIN2

elastin microfibril interfacer 2

2.6

SVF

proper matrix

1292

COL6A2

collagen, type VI, alpha 2

2.24

SVF

cell matrix adhesion

1291

COL6A1

collagen, type VI, alpha 1

1.93*

SVF*

cell matrix adhesion

11117

EMILIN1

elastin microfibril interfacer 1

1.38

SVF

proper matrix

7373

COL14A1

collagen, type XIV, alpha 1 (undulin)

1.36

—

cell matrix adhesion

50509

COL5A3

collagen, type V, alpha 3

1.28

Adipocyte

proper matrix

1302

COL11A2

collagen, type XI, alpha 2

0.78

SVF

proper matrix

1306

COL15A1

collagen, type XV, alpha 1

0.77

SVF

proper matrix

1280

COL2A1

collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital)

0.76

—

proper matrix

1282

COL4A1

collagen, type IV, alpha 1

0.75

SVF**

proper matrix

255631

COL24A1

collagen, type XXIV, alpha 1

0.72

SVF

proper matrix

81578

COL21A1

collagen, type XXI, alpha 1

0.72

SVF

proper matrix

Glycoproteins

6614

SIGLEC1

sialic acid binding Ig-like lectin 1, sialoadhesin

4.13

SVF

cell cell adhesion

7058

THBS2

thrombospondin 2

3.49

SVF

cell matrix adhesion

7148

TNXB

tenascin XB

2.46

SVF

cell matrix adhesion

3959

LGALS3BP

lectin, galactoside-binding, soluble, 3 binding protein

2.26

Adipocyte

cell cell adhesion

10631

POSTN

periostin, osteoblast specific factor

2.14

SVF

cell matrix adhesion

7123

CLEC3B

C-type lectin domain family 3, member B

2.06

SVF

cell cell adhesion

3913

LAMB2

laminin, beta 2 (laminin S)

2

—

7059

THBS3

thrombospondin 3

2

SVF

cell matrix adhesion

4973

OLR1

oxidised low density lipoprotein (lectin-like) receptor 1

1.46

—

cell cell adhesion

1005

CDH7

cadherin 7, type 2

1.41

—

cell cell adhesion

81562

LMAN2L

lectin, mannose-binding 2-like

1.36

SVF

cell cell adhesion

3911

LAMA5

laminin, alpha 5

1.34*

SVF**

cell matrix adhesion

9358

ITGBL1

integrin, beta-like 1 (with EGF-like repeat domains)

1.25

—

cell matrix adhesion

8434

RECK

reversion-inducing-cysteine-rich protein with kazal motifs

1.19

—

remodeling

946

SIGLEC6

sialic acid binding Ig-like lectin 6

0.9

—

cell cell adhesion

4099

MAG

myelin associated glycoprotein

0.89

—

cell matrix adhesion

60437

CDH26

cadherin-like 26

0.85

SVF

cell cell adhesion

1016

CDH18

cadherin 18, type 2

0.8

—

cell cell adhesion

9423

NTN1

netrin 1

0.79

—

57526

PCDH19

protocadherin 19

0.78

SVF

cell cell adhesion

3909

LAMA3

laminin, alpha 3

0.77

Adipocyte

cell matrix adhesion

3910

LAMA4

laminin, alpha 4

0.76

SVF

cell matrix adhesion

3908

LAMA2

laminin, alpha 2 (merosin, congenital muscular dystrophy)

0.73

SVF

cell matrix adhesion

3685

ITGAV

integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

0.72*

Adipocyte**

cell matrix adhesion

11181

TREH

trehalase (brush-border membrane glycoprotein)

0.72

SVF

remodeling

7057

THBS1

thrombospondin 1

0.72*

Adipocyte**

cell matrix adhesion

3691

ITGB4

integrin, beta 4

0.72

—

cell matrix adhesion

3964

LGALS8

lectin, galactoside-binding, soluble, 8 (galectin 8)

0.7

SVF

cell cell adhesion

1012

CDH13

cadherin 13, H-cadherin (heart)

0.7

SVF

cell cell adhesion

1015

CDH17

cadherin 17, LI cadherin (liver-intestine)

0.66

SVF

cell cell adhesion

4974

OMG

oligodendrocyte myelin glycoprotein

0.66

—

cell matrix adhesion

53841

MUCDHL

mucin and cadherin-like

0.63

—

cell cell adhesion

3694

ITGB6

integrin, beta 6

0.6

SVF

cell matrix adhesion

22795

NID2

nidogen 2 (osteonidogen)

0.6

SVF

cell matrix adhesion

2815

GP9

glycoprotein IX (platelet)

0.53

SVF

cell matrix adhesion

3960

LGALS4

lectin, galactoside-binding, soluble, 4 (galectin 4)

0.5

SVF

cell matrix adhesion

1952

CELSR2

cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)

0.5

SVF

cell cell adhesion

6402

SELL

selectin L (lymphocyte adhesion molecule 1)

0.31

SVF

cell cell adhesion

6401

SELE

selectin E (endothelial adhesion molecule 1)

0.28

SVF

cell cell adhesion

Glycosaminoglycans and proteoglycans

51604

PIGT

phosphatidylinositol glycan anchor biosynthesis, class T

2

—

remodeling

1605

DAG1

dystroglycan 1 (dystrophin-associated glycoprotein 1)

1.93

SVF

cell matrix adhesion

145864

HAPLN3

hyaluronan and proteoglycan link protein 3

1.87

—

cell matrix adhesion

9672

SDC3

syndecan 3 (N-syndecan)

1.59

SVF

cell matrix adhesion

93183

PIGM

phosphatidylinositol glycan anchor biosynthesis, class M

1.55

—

remodeling

6383

SDC2

syndecan 2 (heparan sulfate proteoglycan 1, cell surface-associated, fibroglycan)

1.32

—

cell matrix adhesion

6695

SPOCK1

sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1

1.15

SVF

proper matrix

2719

GPC3

glypican 3

0.92

SVF**

cell matrix adhesion

9487

PIGL

phosphatidylinositol glycan anchor biosynthesis, class L

0.91

—

remodeling

8404

SPARCL1

SPARC-like 1 (mast9, hevin)

0.88

SVF

proper matrix

10026

PIGK

phosphatidylinositol glycan anchor biosynthesis, class K

0.85

—

remodeling

127435

PODN

podocan

0.68

—

proper matrix

5281

PIGF

phosphatidylinositol glycan anchor biosynthesis, class F

0.67

—

remodeling

6382

SDC1

syndecan 1

0.63

SVF

cell matrix adhesion

Enzymes

1511

CTSG

cathepsin G

2.75

SVF

remodeling

2990

GUSB

glucuronidase, beta

2.73

SVF

remodeling

2517

FUCA1

fucosidase, alpha-L- 1, tissue

2.62

SVF

remodeling

5351

PLOD1

procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1

2.48

Adipocyte

remodeling

7076

TIMP1

TIMP metallopeptidase inhibitor 1

2.45

SVF

remodeling

3425

IDUA

iduronidase, alpha-L-

2.3

—

remodeling

9956

HS3ST2

heparan sulfate (glucosamine) 3-O-sulfotransferase 2

2.24

SVF

remodeling

5034

P4HB

procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide

2.17

—

remodeling

1509

CTSD

cathepsin D (lysosomal aspartyl peptidase)

2.15

Adipocyte

remodeling

54878

DPP8

dipeptidyl-peptidase 8

2.14

—

remodeling

8985

PLOD3

procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3

2.12

SVF

remodeling

4313

MMP2

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

2.11**

SVF**

remodeling

951

CD37

CD37 molecule

2.04

SVF

remodeling

8869

ST3GAL5

ST3 beta-galactoside alpha-2,3-sialyltransferase 5

2.02

SVF

remodeling

51172

NAGPA

N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

1.94

—

remodeling

30835

CD209

CD209 molecule

1.91

SVF

remodeling

26301

GBGT1

globoside alpha-1,3-N-acetylgalactosaminyltransferase 1

1.89

—

remodeling

3423

IDS

iduronate 2-sulfatase (Hunter syndrome)

1.79

Adipocyte

remodeling

201595

STT3B

STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)

1.78

—

remodeling

64772

FLJ21865

endo-beta-N-acetylglucosaminidase

1.76

SVF

remodeling

25823

TPSG1

tryptase gamma 1

1.74

SVF

remodeling

4249

MGAT5

mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase

1.73

—

remodeling

8722

CTSF

cathepsin F

1.72

Adipocyte

remodeling

60495

HPSE2

heparanase 2

1.72

Adipocyte

remodeling

649

BMP1

bone morphogenetic protein 1

1.72

—

remodeling

5118

PCOLCE

procollagen C-endopeptidase enhancer

1.70

SVF

7077

TIMP2

TIMP metallopeptidase inhibitor 2

1.68

SVF

remodeling

120071

GYLTL1B

glycosyltransferase-like 1B

1.67

—

remodeling

8702

B4GALT4

UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4

1.62

—

remodeling

3073

HEXA

hexosaminidase A (alpha polypeptide)

1.62

SVF

remodeling

9514

GAL3ST1

galactose-3-O-sulfotransferase 1

1.56

SVF

remodeling

222537

HS3ST5

heparan sulfate (glucosamine) 3-O-sulfotransferase 5

1.49

SVF

remodeling

29906

ST8SIA5

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5

1.48

—

remodeling

126792

B3GALT6

UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6

1.46

SVF

remodeling

11253

MAN1B1

mannosidase, alpha, class 1B, member 1

1.45

—

remodeling

54187

NANS

N-acetylneuraminic acid synthase (sialic acid synthase)

1.4

SVF

remodeling

8751

ADAM15

ADAM metallopeptidase domain 15 (metargidin)

1.4

SVF

remodeling

6480

ST6GAL1

ST6 beta-galactosamide alpha-2,6-sialyltranferase 1

1.33

SVF

remodeling

7512

XPNPEP2

X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound

1.31

SVF

remodeling

3026

HABP2

hyaluronan binding protein 2

1.28

Adipocyte

remodeling

7355

SLC35A2

solute carrier family 35 (UDP-galactose transporter), member A2

1.27

—

remodeling

9951

HS3ST4

heparan sulfate (glucosamine) 3-O-sulfotransferase 4

1.26

Adipocyte

remodeling

4185

ADAM11

ADAM metallopeptidase domain 11

1.26

Adipocyte

remodeling

9091

PIGQ

phosphatidylinositol glycan anchor biosynthesis, class Q

1.26

SVF

remodeling

23324

MAN2B2

mannosidase, alpha, class 2B, member 2

1.25

—

remodeling

10585

POMT1

protein-O-mannosyltransferase 1

1.22

—

remodeling

8705

B3GALT4

UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4

1.2

—

remodeling

91039

DPP9

dipeptidyl-peptidase 9

1.18

—

remodeling

6184

RPN1

ribophorin I

1.18

—

remodeling

9486

CHST10

carbohydrate sulfotransferase 10

1.16

—

cell matrix adhesion

55501

CHST12

carbohydrate (chondroitin 4) sulfotransferase 12

1.16

SVF

remodeling

79716

NPEPL1

aminopeptidase-like 1

1.16

Adipocyte

remodeling

9488

PIGB

phosphatidylinositol glycan anchor biosynthesis, class B

1.15

Adipocyte

remodeling

55790

ChGn

chondroitin beta1,4 N-acetylgalactosaminyltransferase

1.14

—

remodeling

411

ARSB

arylsulfatase B

1.12

—

remodeling

11282

MGAT4B

mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B

1.1

Adipocyte

remodeling

115209

OMA1

OMA1 homolog, zinc metallopeptidase (S. cerevisiae)

0.92

—

remodeling

1362

CPD

carboxypeptidase D

0.91

SVF

remodeling

53616

ADAM22

ADAM metallopeptidase domain 22

0.89

SVF

remodeling

5328

PLAU

plasminogen activator, urokinase

0.86

SVF

remodeling

23443

SLC35A3

solute carrier family 35 (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter), member A3

0.86

—

remodeling

8756

ADAM7

ADAM metallopeptidase domain 7

0.87

—

remodeling

6484

ST3GAL4

ST3 beta-galactoside alpha-2,3-sialyltransferase 4

0.85

—

remodeling

11093

ADAMTS13

ADAM metallopeptidase with thrombospondin type 1 motif, 13

0.85

—

cell matrix adhesion

5518

PPP2R1A

protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform

0.85

—

remodeling

8703

B4GALT3

UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3

0.83

SVF

remodeling

7113

TMPRSS2

transmembrane protease, serine 2

0.83

—

remodeling

4323

MMP14

matrix metallopeptidase 14 (membrane-inserted)

0.81

SVF

remodeling

23169

SLC35D1

solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1

0.81

—

remodeling

84750

FUT10

fucosyltransferase 10 (alpha (1,3) fucosyltransferase)

0.79

—

remodeling

5266

PI3

peptidase inhibitor 3, skin-derived (SKALP)

0.79

SVF

remodeling

81849

ST6GALNAC5

ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3) N-acetylgalactosaminide alpha-2,6-sialyltransferase 5

0.78

SVF

remodeling

55454

GALNACT—2

chondroitin sulfate GalNAcT-2

0.77

SVF

remodeling

23213

SULF1

sulfatase 1

0.76

SVF

remodeling

2135

EXTL2

exostoses (multiple)-like 2

0.76

SVF

remodeling

9215

LARGE

like-glycosyltransferase

0.75

SVF

remodeling

4247

MGAT2

mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase

0.74

SVF

remodeling

29929

ALG6

asparagine-linked glycosylation 6 homolog (S. cerevisiae, alpha-1,3-glucosyltransferase)

0.73

—

remodeling

43

ACHE

acetylcholinesterase (Yt blood group)

0.72

—

cell cell adhesion

90161

HS6ST2

heparan sulfate 6-O-sulfotransferase 2

0.73

—

remodeling

8728

ADAM19

ADAM metallopeptidase domain 19 (meltrin beta)

0.72

SVF

remodeling

4316

MMP7

matrix metallopeptidase 7 (matrilysin, uterine)

0.73

SVF

remodeling

4248

MGAT3

mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase

0.71

SVF

remodeling

4320

MMP11

matrix metallopeptidase 11 (stromelysin 3)

0.69

SVF

remodeling

2583

B4GALNT1

beta-1,4-N-acetyl-galactosaminyl transferase 1

0.68

SVF

remodeling

2132

EXT2

exostoses (multiple) 2

0.67

—

remodeling

4322

MMP13

matrix metallopeptidase 13 (collagenase 3)

0.66

—

remodeling

8707

B3GALT2

UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2

0.62

—

remodeling

9427

ECEL1

endothelin converting enzyme-like 1

0.54

—

remodeling

5329

PLAUR

plasminogen activator, urokinase receptor

0.53

SVF

remodeling

9510

ADAMTS1

ADAM metallopeptidase with thrombospondin type 1 motif, 1

0.43

SVF

remodeling

4324

MMP15

matrix metallopeptidase 15 (membrane-inserted)

0.35

—

remodeling

Other components

51148

CEECAM1

cerebral endothelial cell adhesion molecule 1

2.67

Adipocyte

cell cell adhesion

947

CD34

CD34 molecule

2.2

SVF

cell matrix adhesion

2162

F13A1

coagulation factor XIII, A1 polypeptide

2.03

SVF

remodeling

8839

WISP2

WNT1 inducible signaling pathway protein 2

1.94

SVF

cell matrix adhesion

30008

EFEMP2

EGF-containing fibulin-like extracellular matrix protein 2

1.83

Adipocyte

proper matrix

1893

ECM1

extracellular matrix protein 1

1.68

SVF

proper matrix

57530

CGN

cingulin

1.63

—

cell cell adhesion

8642

DCHS1

dachsous 1 (Drosophila)

1.57

SVF

cell matrix adhesion

50636

TMEM16G

transmembrane protein 16G

1.54

Adipocyte

cell matrix adhesion

7301

TYRO3

TYRO3 protein tyrosine kinase

1.53

Adipocyte

cell matrix adhesion

11167

FSTL1

follistatin-like 1

1.32

SVF

cell matrix adhesion

285761

DCBLD1

discoidin, CUB and LCCL domain containing 1

1.3

—

cell cell adhesion

5239

PGM5

phosphoglucomutase 5

1.3

SVF

cell matrix adhesion

53942

CNTN5

contactin 5

1.24

—

cell matrix adhesion

6900

CNTN2

contactin 2 (axonal)

1.24

—

cell matrix adhesion

682

BSG

basigin (Ok blood group)

1.24

Adipocyte

cell matrix adhesion

50848

F11R

F11 receptor

1.22

—

cell cell adhesion

634

CEACAM1

carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)

1.21

—

cell matrix adhesion

6687

SPG7

spastic paraplegia 7, paraplegin (pure and complicated autosomal recessive)

1.17

—

cell matrix adhesion

94030

LRRC4B

leucine rich repeat containing 4B

1.16

Adipocyte

cell matrix adhesion

114990

VASN

vasorin

1.16

SVF

cell matrix adhesion

11047

ADRM1

adhesion regulating molecule 1

1.15

—

cell matrix adhesion

84552

PARD6G

par-6 partitioning defective 6 homolog gamma (C. elegans)

1.11

—

cell cell adhesion

55591

VEZT

vezatin, adherens junctions transmembrane protein

0.89

—

cell cell adhesion

58494

JAM2

junctional adhesion molecule 2

0.86

SVF

cell cell adhesion

1089

CEACAM4

carcinoembryonic antigen-related cell adhesion molecule 4

0.85

SVF

cell cell adhesion

2196

FAT2

FAT tumor suppressor homolog 2 (Drosophila)

0.84

—

cell matrix adhesion

131566

DCBLD2

discoidin, CUB and LCCL domain containing 2

0.84

—

cell matrix adhesion

1366

CLDN7

claudin 7

0.81

SVF

cell cell adhesion

125111

GJC1

gap junction protein, chi 1, 31.9kDa (connexin 31.9)

0.8

SVF

cell matrix adhesion

9019

MPZL1

myelin protein zero-like 1

0.8

SVF

cell cell adhesion

3385

ICAM3

intercellular adhesion molecule 3

0.8

—

cell cell adhesion

9069

CLDN12

claudin 12

0.79

—

cell cell adhesion

5314

PKHD1

polycystic kidney and hepatic disease 1 (autosomal recessive)

0.79

—

cell cell adhesion

9073

CLDN8

claudin 8

0.78

—

cell matrix adhesion

5649

RELN

reelin

0.78

—

cell cell adhesion

4148

MATN3

matrilin 3

0.78

SVF

proper matrix

460

ASTN1

astrotactin 1

0.74

SVF

cell cell adhesion

6091

ROBO1

roundabout, axon guidance receptor, homolog 1 (Drosophila)

0.73

—

cell matrix adhesion

2697

GJA1

gap junction protein, alpha 1, 43kDa (connexin 43)

0.71

SVF

cell cell adhesion

24145

PANX1

pannexin 1

0.7

SVF

cell cell adhesion

3383

ICAM1

intercellular adhesion molecule 1 (CD54), human rhinovirus receptor

0.66

SVF

cell cell adhesion

2152

F3

coagulation factor III (thromboplastin, tissue factor)

0.63

—

remodeling

8174

MADCAM1

mucosal vascular addressin cell adhesion molecule 1

0.61

SVF

cell matrix adhesion

347902

AMIGO2

adhesion molecule with Ig-like domain 2

0.59

SVF

cell cell adhesion

8875

VNN2

vanin 2

0.54

SVF

cell matrix adhesion

7087

ICAM5

intercellular adhesion molecule 5, telencephalin

0.47

Adipocyte

cell cell adhesion

4680

CEACAM6

carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen)

0.43

SVF

cell cell adhesion

SVF = Stroma Vascular Fraction

The indicated fold variations were measured in the cDNA microarray experiments.

* Using RTqPCR, the change in expression of these genes was confirmed in 6 independent obese subjects before and after weight loss induced by gastric bypass.

** Using RTqPCR, the change in expression of these genes was also confirmed in 6 independent samples of human WAT where mature adipocytes and SVF fractions were separated by enzymatic digestion.