Adipose tissue transcriptomic signature highlights the pathologic relevance

of extracellular matrix in human obesity

 

Henegar C, Tordjman J, Achard V, Lacasa D, Cremer I, Guerre-Millo M, Poitou C, Basdevant A,

 Stich V, Viguerie N, Langin D, Bedossa P, Zucker J-D, Clement K.

 

Genome Biology 2008, 9(1):R14

 

Transcriptomic profile of the subcutaneous obese WAT*

  • Clinical and biochemical parameters of the obese subjects and lean controls analyzed in this condition

Clinical and Biochemical Parameters

 

  • Functional analysis of differentially expressed genes in the subcutaneous WAT (obese subjects vs. lean controls)

 

  • Transcriptomic interaction networks constructed from Gene Ontology (GO) or KEGG biological themes annotating differentially expressed genes in the subcutaneous WAT of obese subjects vs. lean controls

 

Functional Networks

Co-expression Networks

GO Cellular Component

GO Biological Process

KEGG

 

  • ECM related genes significantly regulated in the subcutaneous WAT of obese subjects vs. lean controls

ECM Significantly Regulated Genes

Online Table 1:

List of ECM related genes differentially expressed in obese vs. lean condition

 

Co-expression Network

 

 

Shift in the transcriptomic profile of the obese WAT*

three months after gastric bypass

 

  • Clinical and biochemical parameters of the obese subjects analyzed in this condition

Clinical and Biochemical Parameters

 

  • Functional analysis of differentially expressed genes in the subcutaneous WAT of obese subjects after gastric bypass

 

  • Transcriptomic interaction networks constructed from Gene Ontology (GO) or KEGG biological themes annotating differentially expressed genes in the subcutaneous WAT of obese subjects after gastric bypass

 

Functional Networks

Co-expression Networks

GO Cellular Component

GO Biological Process

Whole network

 

Modules 1 & 2

 

Modules 2 & 3

Whole network

Modules 1 & 2

Modules 2 & 3

KEGG

 

  • ECM related genes significantly regulated in the subcutaneous WAT of obese subjects after gastric bypass

ECM Significantly Regulated Genes

Online Table 2:

List of ECM related genes differentially expressed in gastric bypass condition

 

Co-expression Network

 

Transcriptomic profile of human preadipocytes cultured with activated macrophages media

 

  • Functional analysis of differentially expressed genes in preadipocytes cultured with LPS activated macrophages media

 

  • ECM and inflammation related genes significantly induced in preadipocytes cultured with LPS activated macrophages media

ECM and Inflammation Induced Genes

Online Table 3:

List of ECM and inflammation related genes induced in preadipocytes cultured with activated macrophages media

 

 

 

 

Supplementary data on ECM genes

 

 

  • RTqPCR measurements of the expression levels of selected ECM genes in the two main fractions of the subcutaneous WAT in obese subjects and lean controls

 

 

  • Differentially expressed ECM related genes and their expression levels in the three analyzed conditions

List of ECM related genes

 

 

*WAT - human white adipose tissue

 

 

 

Last updated: 22.01.2008

2003 - 2008 by Corneliu Henegar