Adipose tissue transcriptomic signature highlights the pathologic relevance

of extracellular matrix in human obesity

Henegar C, Tordjman J, Achard V, Lacasa D, Cremer I, Guerre-Millo M, Poitou C, Basdevant A,

 Stich V, Viguerie N, Langin D, Bedossa P, Zucker J-D, Clement K.

Genome Biology 2008, 9(1):R14

• Clinical and biochemical parameters of the obese subjects and lean controls analyzed in this condition

Clinical and Biochemical Parameters

• Functional analysis of differentially expressed genes in the subcutaneous WAT (obese subjects vs. lean controls)

• Transcriptomic interaction networks constructed from Gene Ontology (GO) or KEGG biological themes annotating differentially expressed genes in the subcutaneous WAT of obese subjects vs. lean controls

Functional Networks	Co-expression Networks
GO Cellular Component
GO Biological Process
KEGG

 

• ECM related genes significantly regulated in the subcutaneous WAT of obese subjects vs. lean controls

ECM Significantly Regulated Genes
Online Table 1: List of ECM related genes differentially expressed in obese vs. lean condition	Co-expression Network

 

 

Shift in the transcriptomic profile of the obese WAT* three months after gastric bypass

 

• Clinical and biochemical parameters of the obese subjects analyzed in this condition

Clinical and Biochemical Parameters

• Functional analysis of differentially expressed genes in the subcutaneous WAT of obese subjects after gastric bypass

• Transcriptomic interaction networks constructed from Gene Ontology (GO) or KEGG biological themes annotating differentially expressed genes in the subcutaneous WAT of obese subjects after gastric bypass

Functional Networks	Co-expression Networks
GO Cellular Component
GO Biological Process
Whole network   Modules 1 & 2   Modules 2 & 3	Whole network Modules 1 & 2 Modules 2 & 3
KEGG

• ECM related genes significantly regulated in the subcutaneous WAT of obese subjects after gastric bypass

• Functional analysis of differentially expressed genes in preadipocytes cultured with LPS activated macrophages media

• ECM and inflammation related genes significantly induced in preadipocytes cultured with LPS activated macrophages media

• RTqPCR measurements of the expression levels of selected ECM genes in the two main fractions of the subcutaneous WAT in obese subjects and lean controls

• Differentially expressed ECM related genes and their expression levels in the three analyzed conditions

List of ECM related genes

*WAT - human white adipose tissue

Last updated: 22.01.2008

© 2003 - 2008 by Corneliu Henegar